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Generation of helper-free amphotropic retroviruses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene.

We constructed several retroviruses which transduced a mutant dihydrofolate reductase gene that was resistant to methotrexate inhibition and functioned as a dominant selectable marker. The titer of dihydrofolate reductase-transducing virus produced by virus-producing cells could be increased to very...

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Détails bibliographiques
Auteurs principaux: Miller, A D, Law, M F, Verma, I M
Format: Artigo
Langue:Inglês
Publié: 1985
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC366734/
https://ncbi.nlm.nih.gov/pubmed/2985952
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