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Generation of helper-free amphotropic retroviruses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene.

We constructed several retroviruses which transduced a mutant dihydrofolate reductase gene that was resistant to methotrexate inhibition and functioned as a dominant selectable marker. The titer of dihydrofolate reductase-transducing virus produced by virus-producing cells could be increased to very...

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Publicat a:Mol Cell Biol
Autors principals: Miller, A D, Law, M F, Verma, I M
Format: Artigo
Idioma:Inglês
Publicat: Taylor & Francis 1985
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Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC366734/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2985952/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.5.3.431
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