Wird geladen...

In vivo kinetics of protein targeting to the endoplasmic reticulum determined by site-specific phosphorylation

We have developed a novel assay to detect the cytosolic localization of protein domains by inserting a short consensus sequence for phosphorylation by protein kinase A. In transfected COS-1 cells, this sequence was labeled efficiently with [(32)P]phosphate only when exposed to the cytosol and not wh...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:EMBO J
Hauptverfasser: Goder, Veit, Crottet, Pascal, Spiess, Martin
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group 2000
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC366643/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11118205/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/19.24.6704
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!