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In vivo kinetics of protein targeting to the endoplasmic reticulum determined by site-specific phosphorylation
We have developed a novel assay to detect the cytosolic localization of protein domains by inserting a short consensus sequence for phosphorylation by protein kinase A. In transfected COS-1 cells, this sequence was labeled efficiently with [(32)P]phosphate only when exposed to the cytosol and not wh...
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| Publicat a: | EMBO J |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Nature Publishing Group
2000
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC366643/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11118205/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/19.24.6704 |
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