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A single-molecule view of the assembly pathway, subunit stoichiometry and unwinding activity of the bacteriophage T4 primosome (helicase-primase) complex

Single molecule fluorescence resonance energy transfer (smFRET) methods were used to study the assembly pathway and DNA unwinding activity of the bacteriophage T4 helicase-primase (primosome) complex. The helicase substrates used were surface-immobilized model DNA replication forks ‘internally’ labe...

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Detalhes bibliográficos
Main Authors: Lee, Wonbae, Jose, Davis, Phelps, Carey, Marcus, Andrew H., von Hippel, Peter H.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3665360/
https://ncbi.nlm.nih.gov/pubmed/23578280
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi400231s
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