Observation of Solvent Penetration during Cold Denaturation of E. coli Phosphofructokinase-2

Phosphofructokinase-2 is a dimeric enzyme that undergoes cold denaturation following a highly cooperative N(2) [Image: see text] 2I mechanism with dimer dissociation and formation of an expanded monomeric intermediate. Here, we use intrinsic fluorescence of a tryptophan located at the dimer interfac...

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Autores principales: Ramírez-Sarmiento, César A., Baez, Mauricio, Wilson, Christian A.M., Babul, Jorge, Komives, Elizabeth A., Guixé, Victoria
Formato: Artigo
Lenguaje:Inglês
Publicado: The Biophysical Society 2013
Materias:
Proteins and Nucleic Acids
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3660636/
https://ncbi.nlm.nih.gov/pubmed/23708365
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2013.04.024
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