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Observation of Solvent Penetration during Cold Denaturation of E. coli Phosphofructokinase-2
Phosphofructokinase-2 is a dimeric enzyme that undergoes cold denaturation following a highly cooperative N(2) [Image: see text] 2I mechanism with dimer dissociation and formation of an expanded monomeric intermediate. Here, we use intrinsic fluorescence of a tryptophan located at the dimer interfac...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3660636/ https://ncbi.nlm.nih.gov/pubmed/23708365 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2013.04.024 |
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