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Structural and functional roles of Cys-238 and Cys-295 in Escherichia coli phosphofructokinase-2.
Modification of Escherichia coli phosphofructokinase-2 (Pfk-2) with pyrene maleimide (PM) results in a rapid inactivation of the enzyme. The loss of enzyme activity correlates with the incorporation of 2 mol of PM/mol of subunit and the concomitant dissociation of the dimeric enzyme. The two modifie...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2003
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1223755/ https://ncbi.nlm.nih.gov/pubmed/12927023 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20030795 |
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