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Structural and functional roles of Cys-238 and Cys-295 in Escherichia coli phosphofructokinase-2.

Modification of Escherichia coli phosphofructokinase-2 (Pfk-2) with pyrene maleimide (PM) results in a rapid inactivation of the enzyme. The loss of enzyme activity correlates with the incorporation of 2 mol of PM/mol of subunit and the concomitant dissociation of the dimeric enzyme. The two modifie...

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Bibliographic Details
Main Authors: Baez, Mauricio, Rodríguez, Patricio H, Babul, Jorge, Guixé, Victoria
Format: Artigo
Language:Inglês
Published: 2003
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1223755/
https://ncbi.nlm.nih.gov/pubmed/12927023
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20030795
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