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Tagging the Expressed Protein with 6 Histidines: Rapid Cloning of an Amplicon with Three Options
We report the designing of three expression vectors that can be used for rapid cloning of any blunt-end DNA segment. Only a single set of oligonucleotides are required to perform the amplification of the target DNA and its cloning in all three vectors simultaneously. The DNA thus cloned can express...
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| Auteurs principaux: | , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Public Library of Science
2013
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3655076/ https://ncbi.nlm.nih.gov/pubmed/23691118 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0063922 |
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