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Tagging the Expressed Protein with 6 Histidines: Rapid Cloning of an Amplicon with Three Options

We report the designing of three expression vectors that can be used for rapid cloning of any blunt-end DNA segment. Only a single set of oligonucleotides are required to perform the amplification of the target DNA and its cloning in all three vectors simultaneously. The DNA thus cloned can express...

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Detalhes bibliográficos
Principais autores: Singh, Manika Indrajit, Jain, Vikas
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3655076/
https://ncbi.nlm.nih.gov/pubmed/23691118
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0063922
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