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Tagging the Expressed Protein with 6 Histidines: Rapid Cloning of an Amplicon with Three Options

We report the designing of three expression vectors that can be used for rapid cloning of any blunt-end DNA segment. Only a single set of oligonucleotides are required to perform the amplification of the target DNA and its cloning in all three vectors simultaneously. The DNA thus cloned can express...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Singh, Manika Indrajit, Jain, Vikas
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Public Library of Science 2013
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC3655076/
https://ncbi.nlm.nih.gov/pubmed/23691118
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0063922
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