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Barcoding cells using cell-surface programmable DNA-binding domains
We develop here a novel approach to barcode large numbers of cells through cell-surface expression of programmable zinc-finger DNA-binding domains (sZFs). We show sZFs enable double-stranded DNA to sequence-specifically label living cells, and also develop a sequential tagging approach to in situ im...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3641172/ https://ncbi.nlm.nih.gov/pubmed/23503053 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2407 |
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