A two-step enzymatic glycosylation of polypeptides with complex N-glycans

A chemoenyzmatic method for direct glycosylation of polypeptides is described. The method consists of two site-specific enzymatic glycosylation steps: introduction of a glucose moiety at the consensus N-glycosylation sequence (NXS/T) in a polypeptide by an N-glycosyltransferase (NGT) and attachment...

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Hauptverfasser: Lomino, Joseph V., Naegeli, Andreas, Orwenyo, Jared, Amin, Mohammed N., Aebi, Markus, Wang, Lai-Xi
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3638901/
https://ncbi.nlm.nih.gov/pubmed/23477942
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmc.2013.02.007
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