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A two-step enzymatic glycosylation of polypeptides with complex N-glycans
A chemoenyzmatic method for direct glycosylation of polypeptides is described. The method consists of two site-specific enzymatic glycosylation steps: introduction of a glucose moiety at the consensus N-glycosylation sequence (NXS/T) in a polypeptide by an N-glycosyltransferase (NGT) and attachment...
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| Main Authors: | , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3638901/ https://ncbi.nlm.nih.gov/pubmed/23477942 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmc.2013.02.007 |
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