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Increased Sensitivity in Whole-Genome Bisulfite Sequencing (Wgbs): A Novel “Post-bisulfite Conversion” Library Construction Method from Sub-nanogram DNA Inputs

Genome-wide analysis of 5-methylcytosines is possible with whole-genome bisulfite sequencing (WGBS), where unmethylated cytosine residues are converted to uracil. However, a major challenge in WGBS is the degradation of DNA that occurs during bisulfite conversion under conditions required for comple...

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Bibliografske podrobnosti
Main Authors: Hitchen, John, Gabel, Dixie, Caruccio, Nick, Adey, Andrew, Sooknanan, Roy
Format: Artigo
Jezik:Inglês
Izdano: Association of Biomolecular Resource Facilities 2013
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3635258/
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