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Recombinant Expression, Isotope Labeling and Purification of the Vitamin D Receptor Binding Peptide
The vitamin D receptor binding peptide, VDRBP, was overexpressed as a fused form with the ubiquitin molecule in Rosetta(DE3)pLysS, a protein production strain of Escherichia coli harboring an induction controller plasmid. The fusion protein was bound to the immobilized metal ions, and the denaturati...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2011
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3634580/ https://ncbi.nlm.nih.gov/pubmed/23626398 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5012/bkcs.2011.32.12.4337 |
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