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Efficient stable isotope labeling and purification of vitamin D receptor from inclusion bodies

Vitamin D receptor (VDR) plays a crucial role in many cellular processes including calcium and phosphate homeostasis. Previous purification methods from prokaryotic and eukaryotic expression systems were challenged by low protein solubility accompanied by multi purification steps resulting in poor p...

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Detalhes bibliográficos
Main Authors: Zhu, Jinge, Rao, Hongyu, Tonelli, Marco, Westler, Milo, Singarapu, Kiran K., Markley, John L., DeLuca, Hector F., Assadi-Porter, Fariba M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3432171/
https://ncbi.nlm.nih.gov/pubmed/22750673
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2012.06.012
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