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Recombinant Production, Isotope Labeling and Purification of ENOD40B, a Plant Peptide Hormone
The plant peptide hormone ENOD40B was produced in a protein production strain of Escherichia coli harboring an induction controller plasmid (Rosetta(DE3)pLysS) as a His(6)-tagged ubiquitin fusion protein. The fusion protein product was denatured and refolded as part of the isolation procedure and pu...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3542969/ https://ncbi.nlm.nih.gov/pubmed/22702897 |
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