Thermostable Mismatch-Recognizing Protein MutS Suppresses Nonspecific Amplification during Polymerase Chain Reaction (PCR)

Polymerase chain reaction (PCR)-related technologies are hampered mainly by two types of error: nonspecific amplification and DNA polymerase-generated mutations. Here, we report that both errors can be suppressed by the addition of a DNA mismatch-recognizing protein, MutS, from a thermophilic bacter...

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Bibliographic Details
Main Authors: Fukui, Kenji, Bessho, Yoshitaka, Shimada, Atsuhiro, Yokoyama, Shigeyuki, Kuramitsu, Seiki
Format: Artigo
Language:Inglês
Published: Molecular Diversity Preservation International (MDPI) 2013
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3634412/
https://ncbi.nlm.nih.gov/pubmed/23519109
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms14036436
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