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Thermostable Mismatch-Recognizing Protein MutS Suppresses Nonspecific Amplification during Polymerase Chain Reaction (PCR)

Polymerase chain reaction (PCR)-related technologies are hampered mainly by two types of error: nonspecific amplification and DNA polymerase-generated mutations. Here, we report that both errors can be suppressed by the addition of a DNA mismatch-recognizing protein, MutS, from a thermophilic bacter...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Fukui, Kenji, Bessho, Yoshitaka, Shimada, Atsuhiro, Yokoyama, Shigeyuki, Kuramitsu, Seiki
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Molecular Diversity Preservation International (MDPI) 2013
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3634412/
https://ncbi.nlm.nih.gov/pubmed/23519109
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms14036436
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