Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.

We developed a method for immunoaffinity purification of Saccharomyces cerevisiae adenylyl cyclase based on creating a fusion with a small peptide epitope. Using oligonucleotide technology to encode the peptide epitope we constructed a plasmid that expressed the fusion protein from the S. cerevisiae...

Celý popis

Uloženo v:
Podrobná bibliografie
Hlavní autoři: Field, J, Nikawa, J, Broek, D, MacDonald, B, Rodgers, L, Wilson, I A, Lerner, R A, Wigler, M
Médium: Artigo
Jazyk:Inglês
Vydáno: 1988
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC363397/
https://ncbi.nlm.nih.gov/pubmed/2455217
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!

Podobné jednotky