Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.

We developed a method for immunoaffinity purification of Saccharomyces cerevisiae adenylyl cyclase based on creating a fusion with a small peptide epitope. Using oligonucleotide technology to encode the peptide epitope we constructed a plasmid that expressed the fusion protein from the S. cerevisiae...

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Pubblicato in:	Mol Cell Biol
Autori principali:	Field, J, Nikawa, J, Broek, D, MacDonald, B, Rodgers, L, Wilson, I A, Lerner, R A, Wigler, M
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Taylor & Francis 1988
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC363397/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2455217/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.8.5.2159-2165.1988
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