Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.

We developed a method for immunoaffinity purification of Saccharomyces cerevisiae adenylyl cyclase based on creating a fusion with a small peptide epitope. Using oligonucleotide technology to encode the peptide epitope we constructed a plasmid that expressed the fusion protein from the S. cerevisiae...

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Detalhes bibliográficos
Main Authors: Field, J, Nikawa, J, Broek, D, MacDonald, B, Rodgers, L, Wilson, I A, Lerner, R A, Wigler, M
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC363397/
https://ncbi.nlm.nih.gov/pubmed/2455217
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