Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.
We developed a method for immunoaffinity purification of Saccharomyces cerevisiae adenylyl cyclase based on creating a fusion with a small peptide epitope. Using oligonucleotide technology to encode the peptide epitope we constructed a plasmid that expressed the fusion protein from the S. cerevisiae...
में बचाया:
| में प्रकाशित: | Mol Cell Biol |
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| मुख्य लेखकों: | , , , , , , , |
| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
Taylor & Francis
1988
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC363397/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2455217/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.8.5.2159-2165.1988 |
| टैग: |
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