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New Protein Footprinting: Fast Photochemical Iodination Combined with Top-down and Bottom-up Mass Spectrometry

We report a new approach for the FPOP (fast photochemical oxidation of proteins) whereby iodine species are used as the modifying reagent. We generate the radicals by photolysis of iodobenzoic acid at 248 nm; the putative iodine radical then rapidly modifies the target protein. This iodine-radical l...

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Detalhes bibliográficos
Main Authors: Chen, Jiawei, Cui, Weidong, Giblin, Daryl, Gross, Michael L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3630512/
https://ncbi.nlm.nih.gov/pubmed/22669760
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13361-012-0403-1
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