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New Protein Footprinting: Fast Photochemical Iodination Combined with Top-down and Bottom-up Mass Spectrometry
We report a new approach for the FPOP (fast photochemical oxidation of proteins) whereby iodine species are used as the modifying reagent. We generate the radicals by photolysis of iodobenzoic acid at 248 nm; the putative iodine radical then rapidly modifies the target protein. This iodine-radical l...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2012
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3630512/ https://ncbi.nlm.nih.gov/pubmed/22669760 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13361-012-0403-1 |
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