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Structural Evidence of a Productive Active Site Architecture for an Evolved Quorum-quenching GKL Lactonase
The in vitro evolution and engineering of quorum-quenching lactonases with enhanced reactivities was achieved using a thermostable GKL enzyme as template, yielding the E101G/R230C GKL mutant with increased catalytic activity and broadened substrate range [Chow, J. Y., Xue, B., Lee, K. H., Tung, A.,...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3629905/ https://ncbi.nlm.nih.gov/pubmed/23461395 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi4000904 |
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