Defining the sequence specificity of DNA-binding proteins by selecting binding sites from random-sequence oligonucleotides: analysis of yeast GCN4 protein.

We describe a new method for accurately defining the sequence recognition properties of DNA-binding proteins by selecting high-affinity binding sites from random-sequence DNA. The yeast transcriptional activator protein GCN4 was coupled to a Sepharose column, and binding sites were isolated by passi...

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Pubblicato in:	Mol Cell Biol
Autori principali:	Oliphant, A R, Brandl, C J, Struhl, K
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Taylor & Francis 1989
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC362762/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2674675/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.9.7.2944-2949.1989
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