Defining the sequence specificity of DNA-binding proteins by selecting binding sites from random-sequence oligonucleotides: analysis of yeast GCN4 protein.
We describe a new method for accurately defining the sequence recognition properties of DNA-binding proteins by selecting high-affinity binding sites from random-sequence DNA. The yeast transcriptional activator protein GCN4 was coupled to a Sepharose column, and binding sites were isolated by passi...
সংরক্ষণ করুন:
| প্রকাশিত: | Mol Cell Biol |
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| প্রধান লেখক: | , , |
| বিন্যাস: | Artigo |
| ভাষা: | Inglês |
| প্রকাশিত: |
Taylor & Francis
1989
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| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC362762/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2674675/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.9.7.2944-2949.1989 |
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