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Mapping in vivo topoisomerase I sites on simian virus 40 DNA: asymmetric distribution of sites on replicating molecules.

Complexes between simian virus 40 DNA and topoisomerase I (topo I) were isolated from infected cells treated with camptothecin. The topo I break sites were precisely mapped by primer extension from defined oligonucleotides. Of the 56 sites, 40 conform to the in vitro consensus sequence previously de...

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Autors principals: Porter, S E, Champoux, J J
Format: Artigo
Idioma:Inglês
Publicat: 1989
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC362630/
https://ncbi.nlm.nih.gov/pubmed/2540421
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