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Development and Validation of a High-density Fluorescence Polarization-based Assay for the Trypanosoma RNA Triphosphatase TbCet1

RNA triphosphatases are attractive and mostly unexplored therapeutic targets for the development of broad spectrum antiprotozoal, antiviral and antifungal agents. The use of malachite green as a readout for phosphatases is well characterized and widely employed. However, the reaction depends on high...

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Detalhes bibliográficos
Main Authors: Antczak, Christophe, Shum, David, Radu, Constantin, Seshan, Venkatraman E., Djaballah, Hakim
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3626118/
https://ncbi.nlm.nih.gov/pubmed/19275531
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