Introduction of functional artificial introns into the naturally intronless ura4 gene of Schizosaccharomyces pombe.

Insertion of a 36-base-pair (bp) synthetic oligonucleotide comprising the sequence 5'-GTAGGT(19N)CTAAT (4N)AG-3' into several different positions within the coding region of the naturally intronless ura4 gene of Schizosaccharomyces pombe leads to an efficiently spliced gene producing a fun...

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Bibliografiske detaljer
Main Authors: Gatermann, K B, Hoffmann, A, Rosenberg, G H, Käufer, N F
Format: Artigo
Sprog:Inglês
Udgivet: 1989
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC362569/
https://ncbi.nlm.nih.gov/pubmed/2725514
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