Introduction of functional artificial introns into the naturally intronless ura4 gene of Schizosaccharomyces pombe.

Insertion of a 36-base-pair (bp) synthetic oligonucleotide comprising the sequence 5'-GTAGGT(19N)CTAAT (4N)AG-3' into several different positions within the coding region of the naturally intronless ura4 gene of Schizosaccharomyces pombe leads to an efficiently spliced gene producing a functional pr...

Disgrifiad llawn

Manylion Llyfryddiaeth

Cyhoeddwyd yn:	Mol Cell Biol
Prif Awduron:	Gatermann, K B, Hoffmann, A, Rosenberg, G H, Käufer, N F
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	Taylor & Francis 1989
Pynciau:	Research Article
Mynediad Ar-lein:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC362569/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2725514/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.9.4.1526-1535.1989
Tagiau:	Ychwanegu Tag Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!