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Introduction of functional artificial introns into the naturally intronless ura4 gene of Schizosaccharomyces pombe.
Insertion of a 36-base-pair (bp) synthetic oligonucleotide comprising the sequence 5'-GTAGGT(19N)CTAAT (4N)AG-3' into several different positions within the coding region of the naturally intronless ura4 gene of Schizosaccharomyces pombe leads to an efficiently spliced gene producing a fun...
Enregistré dans:
| Publié dans: | Mol Cell Biol |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Taylor & Francis
1989
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC362569/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2725514/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.9.4.1526 |
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