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Introduction of functional artificial introns into the naturally intronless ura4 gene of Schizosaccharomyces pombe.

Insertion of a 36-base-pair (bp) synthetic oligonucleotide comprising the sequence 5'-GTAGGT(19N)CTAAT (4N)AG-3' into several different positions within the coding region of the naturally intronless ura4 gene of Schizosaccharomyces pombe leads to an efficiently spliced gene producing a functional pr...

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Detalles Bibliográficos
Publicado en:Mol Cell Biol
Principais autores: Gatermann, K B, Hoffmann, A, Rosenberg, G H, Käufer, N F
Formato: Artigo
Idioma:Inglês
Publicado: Taylor & Francis 1989
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC362569/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2725514/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.9.4.1526-1535.1989
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