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Phosphine-Quenching of Cyanine Dyes as a Versatile Tool for Fluorescence Microscopy

We report that the cyanine dye Cy5 and several of its structural relatives are reversibly quenched by the phosphine TCEP (tris(2-carboxyethyl)phosphine). Using Cy5 as a model, we show that the quenching reaction occurs by 1,4-addition of the phosphine to the polymethine bridge of Cy5 to form a coval...

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Autori principali: Vaughan, Joshua C., Dempsey, Graham T., Sun, Eileen, Zhuang, Xiaowei
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3624894/
https://ncbi.nlm.nih.gov/pubmed/23311875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja3105279
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