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Phosphine-Quenching of Cyanine Dyes as a Versatile Tool for Fluorescence Microscopy
We report that the cyanine dye Cy5 and several of its structural relatives are reversibly quenched by the phosphine TCEP (tris(2-carboxyethyl)phosphine). Using Cy5 as a model, we show that the quenching reaction occurs by 1,4-addition of the phosphine to the polymethine bridge of Cy5 to form a coval...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3624894/ https://ncbi.nlm.nih.gov/pubmed/23311875 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja3105279 |
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