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S1 and KH Domains of Polynucleotide Phosphorylase Determine the Efficiency of RNA Binding and Autoregulation

To better understand the roles of the KH and S1 domains in RNA binding and polynucleotide phosphorylase (PNPase) autoregulation, we have identified and investigated key residues in these domains. A convenient pnp::lacZ fusion reporter strain was used to assess autoregulation by mutant PNPase protein...

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Bibliographische Detailangaben
Hauptverfasser: Wong, Alexander G., McBurney, Kristina L., Thompson, Katharine J., Stickney, Leigh M., Mackie, George A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3624587/
https://ncbi.nlm.nih.gov/pubmed/23457244
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00062-13
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