Analysis of pre-rRNAs in heat-shocked HeLa cells allows identification of the upstream termination site of human polymerase I transcription.
Human rRNA precursor from normal or stressed HeLa cells were studied by S1 nuclease mapping of unlabeled RNA and by antisense RNase mapping of RNA from cells that had been labeled in vivo with [32P]PO4. Heating cells to 43 degrees C decreased the amount of newly synthesized rRNA to less than 5% of t...
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| Published in: | Mol Cell Biol |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Taylor & Francis
1989
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC362323/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2761537/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.9.6.2500-2512.1989 |
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