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Ouabain-resistant mutants of the rat Na,K-ATPase alpha 2 isoform identified by using an episomal expression vector.

Site-directed mutagenesis was used to identify residues responsible for the greater than 1,000-fold difference in ouabain sensitivity between the rat Na,K-ATPase alpha 1 and alpha 2 isoforms. A series of mutagenized cDNAs was constructed that replaced residues of the rat alpha 2 subunit with the cor...

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Détails bibliographiques
Publié dans:Mol Cell Biol
Auteurs principaux: Canfield, V, Emanuel, J R, Spickofsky, N, Levenson, R, Margolskee, R F
Format: Artigo
Langue:Inglês
Publié: Taylor & Francis 1990
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC362238/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2157137/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.10.4.1367
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