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Efficient and specific gene knockdown by small interfering RNAs produced in bacteria
Synthetic small interfering RNAs (siRNAs) are an indispensable tool to investigate gene function in eukaryotic cells(1,2) and may be used for therapeutic purposes to knockdown genes implicated in disease(3). Thus far, most synthetic siRNAs have been produced by chemical synthesis. Here we present a...
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| Autors principals: | , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2013
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3622153/ https://ncbi.nlm.nih.gov/pubmed/23475073 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.2537 |
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