A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.

Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functio...

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izdano v:	Mol Cell Biol
Principais autores:	Winey, M, Edelman, I, Culbertson, M R
Format:	Artigo
Jezik:	Inglês
Izdano:	Taylor & Francis 1989
Teme:	Research Article
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC362177/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2648132/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.9.1.329-331.1989
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