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A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.

Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functio...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Winey, M, Edelman, I, Culbertson, M R
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 1989
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC362177/
https://ncbi.nlm.nih.gov/pubmed/2648132
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