A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo.
Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functio...
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| Publicat a: | Mol Cell Biol |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Taylor & Francis
1989
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC362177/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2648132/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.9.1.329-331.1989 |
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