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Activity-enhancing mutations in an E3 ubiquitin ligase identified by high-throughput mutagenesis

Although ubiquitination plays a critical role in virtually all cellular processes, mechanistic details of ubiquitin (Ub) transfer are still being defined. To identify the molecular determinants within E3 ligases that modulate activity, we scored each member of a library of nearly 100,000 protein var...

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Detalhes bibliográficos
Main Authors: Starita, Lea M., Pruneda, Jonathan N., Lo, Russell S., Fowler, Douglas M., Kim, Helen J., Hiatt, Joseph B., Shendure, Jay, Brzovic, Peter S., Fields, Stanley, Klevit, Rachel E.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2013
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3619334/
https://ncbi.nlm.nih.gov/pubmed/23509263
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1303309110
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