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Massively Parallel Single Amino Acid Mutagenesis
Random mutagenesis methods only partially cover the mutational space, and are constrained by DNA synthesis length limitations. Here, we demonstrate PALS, a single-volume, site-directed mutagenesis approach using microarray-programmed oligonucleotides. We created libraries including nearly every miss...
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| 出版年: | Nat Methods |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2015
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4344410/ https://ncbi.nlm.nih.gov/pubmed/25559584 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3223 |
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