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High-throughput fluorescent-based NKCC functional assay in adherent epithelial cells

BACKGROUND: The kidney-specific NKCC cotransporter isoform NKCC2 is involved in the Na(+) reabsorption in the Thich Ascending Limb (TAL) cells and in the regulation of body fluid volume. In contrast, the isoform NKCC1 represents the major pathway for Cl(-) entry in endothelial cells, playing a cruci...

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Detalhes bibliográficos
Main Authors: Carmosino, Monica, Rizzo, Federica, Torretta, Silvia, Procino, Giuseppe, Svelto, Maria
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3618206/
https://ncbi.nlm.nih.gov/pubmed/23506056
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2121-14-16
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