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High-Throughput Assays to Measure Intracellular Ca(2+) Mobilization in Cells that Express Recombinant S1P Receptor Subtypes
Intracellular Ca(2+) mobilization is a useful readout to screen for agonists or antagonists of G-protein coupled receptors (GPCRs). Here, we describe methods to conduct high-throughput screening of stably or transiently transfected HTC4 cells expressing the individual S1P1–5 receptor subtypes. The c...
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| Главные авторы: | , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2012
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3617928/ https://ncbi.nlm.nih.gov/pubmed/22528441 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-800-9_7 |
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