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Purification, crystallization and preliminary crystallographic analysis of a 4-thiouridine synthetase–RNA complex

The sulfurtransferase 4-thiouridine synthetase (ThiI) is involved in the ATP-dependent modification of U8 in tRNA. ThiI from Thermotoga maritima was cloned, overexpressed and purified. A complex comprising ThiI and a truncated tRNA was prepared and crystallized, and X-ray diffraction data were colle...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Naumann, Peter-Thomas, Lauhon, Charles T., Ficner, Ralf
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: International Union of Crystallography 2013
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3614169/
https://ncbi.nlm.nih.gov/pubmed/23545650
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113004077
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