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Purification, crystallization and preliminary crystallographic analysis of a 4-thiouridine synthetase–RNA complex
The sulfurtransferase 4-thiouridine synthetase (ThiI) is involved in the ATP-dependent modification of U8 in tRNA. ThiI from Thermotoga maritima was cloned, overexpressed and purified. A complex comprising ThiI and a truncated tRNA was prepared and crystallized, and X-ray diffraction data were colle...
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| Hoofdauteurs: | , , |
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| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
International Union of Crystallography
2013
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3614169/ https://ncbi.nlm.nih.gov/pubmed/23545650 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113004077 |
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