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Expedient synthesis of electronically modified luciferins for bioluminescence imaging

Bioluminescence imaging with luciferase enzymes requires access to light-emitting, small molecule luciferins. Here, we describe a rapid method to synthesize d-luciferin, the substrate for firefly luciferase (Fluc), along with a novel set of electronically modified analogs. Our procedure utilizes a r...

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Hlavní autoři: McCutcheon, David C., Paley, Miranda A., Steinhardt, Rachel C., Prescher, Jennifer A.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2012
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3613990/
https://ncbi.nlm.nih.gov/pubmed/22519459
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja301493d
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