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Induction of transformation and DNA synthesis after microinjection of raf proteins.

Full-length and N-terminal deletion mutants of human c-raf-1 cDNA were cloned into Escherichia coli expression plasmids. Bacterially expressed c-raf proteins were purified by anion-exchange, gel filtration, and affinity chromatography. Microinjection of mutant c-raf proteins into G0-arrested NIH 3T3...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Smith, M R, Heidecker, G, Rapp, U R, Kung, H F
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1990
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC360849/
https://ncbi.nlm.nih.gov/pubmed/2192265
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