Induction of transformation and DNA synthesis after microinjection of raf proteins.

Full-length and N-terminal deletion mutants of human c-raf-1 cDNA were cloned into Escherichia coli expression plasmids. Bacterially expressed c-raf proteins were purified by anion-exchange, gel filtration, and affinity chromatography. Microinjection of mutant c-raf proteins into G0-arrested NIH 3T3...

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Detalhes bibliográficos

Publicado no:	Mol Cell Biol
Principais autores:	Smith, M R, Heidecker, G, Rapp, U R, Kung, H F
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Taylor & Francis 1990
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC360849/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2192265/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.10.7.3828-3833.1990
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