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Identification of a 42-kilodalton phosphotyrosyl protein as a serine(threonine) protein kinase by renaturation.

We have surveyed fibroblast lysates for protein kinases that might be involved in mitogenesis. The assay we have used exploits the ability of blotted, sodium dodecyl sulfate-denatured proteins to regain enzymatic activity after guanidine treatment. About 20 electrophoretically distinct protein kinas...

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Библиографические подробности
Опубликовано в: :Mol Cell Biol
Главные авторы: Ferrell, J E, Martin, G S
Формат: Artigo
Язык:Inglês
Опубликовано: Taylor & Francis 1990
Предметы:
Online-ссылка:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC360666/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1692963/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.10.6.3020
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