Identification of a 42-kilodalton phosphotyrosyl protein as a serine(threonine) protein kinase by renaturation.
We have surveyed fibroblast lysates for protein kinases that might be involved in mitogenesis. The assay we have used exploits the ability of blotted, sodium dodecyl sulfate-denatured proteins to regain enzymatic activity after guanidine treatment. About 20 electrophoretically distinct protein kinas...
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| Publicado no: | Mol Cell Biol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC360666/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1692963/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.10.6.3020-3026.1990 |
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